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KMID : 0369820000300040279
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Jorunal of Korean Pharmaceutical Sciences
2000 Volume.30 No. 4 p.279 ~ p.282
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Quantitation of Mevinolinic Acid in Human Plasma by HPLC
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오한석/Oh HS
박동영/서성훈/김영관/홍선표/최영욱/이경태/Park DY/Seo SH/Kim YG/Hong SP/Choi YW/Lee KT
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Abstract
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Simple and precise high-performance liquid chromatographic (HPLC) assay was developed and validated for the determination of a HMG-CoA reductase inhibitor, lovastatinTM and its active metabolite (mevinolinic acid) in human plasma. The method involved solid phase extraction of mevinolinic acid and internal standard using Sep-Pak Cartridge. Samples were analyzed by reversed-phase HPLC using Capcell?PakC18 column with ultraviolet detection at 238 nm. The quantitation limit of mevinolinic acid was 2 ng/ml and the calibration curve was linear over the range of 2-50 ng/ml (r2>0.999) with human plasma. The analyses of quality control samples indicated that the normal values could be predicted with an accuracy >97%. The intra- and inter-day coefficients of variation for the analyses were <10%. The average recoveries were similar (79%) for mevinolinic acid and methylmevinolinic acid. The method described has been successfully applied to the quantification of mevinolinic acid in about 1,000 human plasma samples over six-month period.
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KEYWORD
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Validation, Mevinolinic acid, HPLC, Bioavailability
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